ABSTRACT
Background: MicroRNAs [miRNAs] are implicated in various biological processes including anticoagulation. However, the modulation of miRNA by pharmacological intervention such as warfarin treatment in patients receiving warfarin has not been disclosed yet. The aim of this study work was to assess the effect of warfarin drug on expression level of mir-133a-3p in patients with mechanical heart valve replacement
Methods: In this research, the expression level of miRNA-133a-3p was analyzed in Peripheral Blood Mononuclear cells [PBMCs] from mechanical valve replacement patients who had received warfarin for at least 3 months continuously. Quantitative RT-PCR method was used for this assay
Results: Our findings indicated a significant diffrence between the rate of miR-133a-3p expression in individuals receiving warfarin and the control group [p<0.01]. There was also a statistically significant difference in miR-133a-3p expression in patients with different ages [p<0.05] suggesting that the rate of miR-133a-3p expression in persons receiving warfarin is related to age. However, other variables like warfarin dose, International Normalized Ratio [INR], gender, and Body Mass Index [BMI] were not significantly effective on the miR-133a-3p experssion rate in individuals receving warfarin
Conclusion: Based on our results, it can be concluded that miR-133a-3p is involved in the coagulation pathway. The recent result indicates that warfarin affects the expression of miR-133a. This expression may be potentially important for treatment by anticoagulants. Awareness of the time course of miRNA expression profile can improve efficiency of response to warfarin
ABSTRACT
Studies have indicated that thrombophilic genes polymorphisms are associated with recurrent pregnancy loss [RPL] in the Iranian population. We aimed to evaluate the precise association between thrombophilic genes polymorphisms [MTHFR C677T, MTHFR A1298C, Prothrombin G20210A, FVL G1691A, and PAI-1 4G/5G] and RPL risk in the Iranian population. PubMed, Web of Science, Google Scholar, and ISC were searched for eligible articles published up to April 1, 2017. In total, 37 case-control studies in 18 relevant publications were selected: 1,199, 1,194, 630, 830, and 955 RPL cases and 1,079, 1079, 594, 794, and 499 controls for MTHFR C677T, MTHFR A1298C,Prothrombin G20210A, FVL G1691A, and PAI-1 4G/5G, respectively. The results indicated a significant increased risk of RPL in all genetic models in the population. Also, Prothrombin G20210A and FVL G1691A as well as PAI-1 4G/5G polymorphisms were associated with RPL risk in the Iranian population. Hence, thrombophilic genes polymorphisms are associated with an increased RPL risk in the Iranian population
Subject(s)
Humans , Female , Thrombophilia/genetics , Prothrombin , Polymorphism, GeneticABSTRACT
Alpinia galanga [A. galanga] belongs to the Zingiberaceae family has anti-oxidant effects in animals and humans body and often is used as medicament or part of medicaments in Asian folk medicine for various applications. The objective of this study was to investigate the molecular and biochemical influence of alcoholic extract from the rhizomes of A. galangal on the spermatogenesis process in male rat. Forty five Wistar male rats were divided into three groups, control [n=15] and two tested groups [n=30]. Alcoholic extract [5%] of plant was given by oral route at doses of 100 and 300 mg/day for 56 days and spermatogenesis parameters, hormone changes and expression level of the cAMP-responsive element modulator [CREM] gene were assessed. Methanol extract of A. galanga increased serum testosterones level significantly in both treated groups in comparison with control group [p<0.05]. Besides, the percentage of sperm viability and motility in both tested groups were significantly increased. Follicle stimulating hormone FSH hormone, morphology and weight were affected in both treated groups. With 300 mg/day an increase in sperm count was observed. Sperm motility was increased in two treated groups whereas testis weight was decreased in treated groups. Real time analysis of treated cells of testis showed increase level of mRNA related to CREM gene involved in spermatogenesis process after 56 days induction. It is concluded that application of ethanolic extract of A. galangal significantly increased sperm percentage, viability, motility and testosterone hormone. This suggested that this plant may be promising in enhancing sperm healthy parameters
Subject(s)
Animals, Laboratory , Spermatogenesis , Rats, Wistar , Spermatozoa , Testosterone , Methanol , TestisABSTRACT
Background and aim: Infectious diseases are one of the most important agents of mortality in the world. Due to resistant of some human infectious bacteria to antibiotics, use of plant pharmaceutical herbs to control of these agents is increasing. The aim of this research was to investigate the antibacterial effect of aqueous extract of Yarrow and Salvia against ten bacterial strains of human pathogenic bacteria.
Materials and Methods: Aqueous extract from Achillea millefolium and Teucrium polium species were prepared using maceration method and then were concentrated and dried using rotary apparatus. . Ten standard bacterial strains were cultured on Nutrient Broth. Minimum Inhibitory Concentration [MIC] and Minimum Bactericidal Concentration [MBC] of plant extract of both extract on bacteria were determined using micro dilution method at four different concentrations [0/62-1/25-2/5-5-10mg/ml].
Results:The results showed that aqueous extract from Achillea millefolium and Teucrium polium species have an inhibition effect on all tested bacteria. Staphylococcus aureus and S. saprophyticus showed the highest and lowest sensitivity to water extract of yarrow respectively. Pseudomonas aeroginosa sereus strain showed high degree of sensitivity whereas S. aureuss and Bacillus aeroginosa strains were less sensitive to extract of salvia.
Conclusion: According to obtained results in this work and other researches in this domain, the Aqueous extracts of Yarraw and Salvia could be introduced as a biocontrol agent against pathogenic micro-organisms. Nevertheless to use each extract of plant for clinical application, chemical analysis of extracts and clinical researches are necessary.